IE

Regarding long-term cryopreservation of cells, there are two optional containers, liquid nitrogen tanks and gas-phase liquid nitrogen tanks.

The former is to freeze cells in liquid nitrogen; the latter is to freeze cells in a low-temperature environment generated by liquid nitrogen;

Both of them also rely on liquid nitrogen for cooling, but some people say that using gas-phase liquid nitrogen tanks is safer. Why is this?

In fact, no product can be guaranteed to be perfect. It cannot be said that using gas-phase liquid nitrogen tanks to freeze cells is safer. It can only be said that the safety index is higher than that of conventional liquid nitrogen tanks.

Mainly manifested in three aspects:

1. Storage mode security

The gas-phase storage mode is used to freeze cells, which uses low-temperature nitrogen volatilized by liquid nitrogen for cryopreservation. There is no contact between cells and liquid nitrogen, which can avoid cross-infection of cells during storage and avoid accidental entry of liquid nitrogen into the cryopreservation tube. pipe burst problem;

2. Temperature safety

The temperature inside the gas-phase liquid nitrogen tank is divided into zones. The temperature difference between the upper and lower parts is about 10°C, which can be said to be very stable. The temperature at the mouth of the tank can be maintained at -190°C. The lower the temperature, the lower the temperature. No matter where the cells are stored, , the activity will not be "excited" due to large fluctuations in temperature, thereby losing activity.

3. “Equipment” security

The gas phase liquid nitrogen tank has a complete intelligent monitoring system.

It can detect temperature, 3 temperature sensors, transmit internal temperature in real time;

It can monitor the liquid level, determine the liquid level height, and can pre-set high/low liquid level alarms;

In addition to these functions, there are also heat exhaust functions, lid opening and defogging functions, alarm and remote alarm functions, event recording, and communication functions. These functions all serve the safety of cells.

This is why the safety index of gas-phase liquid nitrogen tanks is higher than that of conventional liquid nitrogen tanks.